Austin, Leland, Milo, and Ruby appeared in 17 strips of a 1977 storyline in which Charlie Brown ran away from home to flee the United States Environmental Protection Agency after taking revenge on a Kite-Eating Tree. [*]Seriously, I think this is right up there with the time Garfield died.
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EDITED TO ADD: Here, in order to scrub those ideas out of your brain - an ALPHABET OF AWESOME. (Via)